The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits ENaC through an increase in the intracellular Cl− concentration
Open Access
- 1 November 2001
- journal article
- research article
- Published by Springer Nature in EMBO Reports
- Vol. 2 (11) , 1047-1051
- https://doi.org/10.1093/embo-reports/kve232
Abstract
Activation of the CFTR Cl− channel inhibits epithelial Na+ channels (ENaC), according to studies on epithelial cells and overexpressing recombinant cells. Here we demonstrate that ENaC is inhibited during stimulation of the cystic fibrosis transmembrance conductance regulator (CFTR) in Xenopus oocytes, independent of the experimental set‐up and the magnitude of the whole‐cell current. Inhibition of ENaC is augmented at higher CFTR Cl− currents. Similar to CFTR, ClC‐0 Cl− currents also inhibit ENaC, as well as high extracellular Na+ and Cl−in partially permeabilized oocytes. Thus, inhibition of ENaC is not specific to CFTR and seems to be mediated by Cl−.Keywords
This publication has 26 references indexed in Scilit:
- Epithelial Sodium Channels Regulate Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channels in XenopusOocytesJournal of Biological Chemistry, 2000
- Feedback inhibition of epithelial Na+ channels in Xenopus oocytes does not require G0 or Gi2 proteinsFEBS Letters, 1999
- The amiloride-inhibitable Na+ conductance is reduced by the cystic fibrosis transmembrane conductance regulator in normal but not in cystic fibrosis airways.Journal of Clinical Investigation, 1998
- Mutations causing Liddle syndrome reduce sodium-dependent downregulation of the epithelial sodium channel in the Xenopus oocyte expression system.Journal of Clinical Investigation, 1998
- Cl− transport by cystic fibrosis transmembrane conductance regulator (CFTR) contributes to the inhibition of epithelial Na+ channels (ENaCs) in Xenopus oocytes co‐expressing CFTR and ENaCThe Journal of Physiology, 1998
- Inhibition of epithelial Na+ currents by intracellular domains of the cystic fibrosis transmembrane conductance regulatorFEBS Letters, 1997
- Regulation of Epithelial Sodium Channels by the Cystic Fibrosis Transmembrane Conductance RegulatorPublished by Elsevier ,1996
- Wild type but not ΔF508 CFTR inhibits Na+ conductance when coexpressed in Xenopus oocytesFEBS Letters, 1996
- CFTR as a cAMP-Dependent Regulator of Sodium ChannelsScience, 1995
- Low access resistance perforated patch recordings using amphotericin BJournal of Neuroscience Methods, 1991