Overcoming Self-incompatibility in Raphanus sativus L. with High Temperature1

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Abstract
Self-incompatibility of Raphanus sativus L. was partially overcome by exposing plants to temperature of 30 to 50°C. The most effective treatment was 50°C for 25 minutes. Scanning electron microscopical (SEM) observation of pollen tubes on the papillae surface have shown that exposure of the gynoecium to 50°C for 25 minutes resulted in pollen tube growth following self-pollination that resembled that of cross-pollination. Openings of papillae and detached pollen grains and tubes were found as the result of successful pollen tube penetration of papillae. Fluorescence microscopical (FM) observation served to confirm these observations made by SEM. However, incompatible pollen failed to germinate although pollen grains were attached to the papillae by aid of their waxy surface substances.

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