Changes in the sialylglycoconjugate distribution on the human sperm surface during in-vitro capacitation: partial purification of a 20 kDa sialylglycoprotein of capacitated spermatozoa
- 1 October 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Human Reproduction
- Vol. 10 (10) , 2755-2759
- https://doi.org/10.1093/oxfordjournals.humrep.a135787
Abstract
Changes in the distribution of sialylglycoconjugates on the surface of uncapacitated and in-vitro capacitated human spermatozoa were studied by means of two sialic acid specific lectins [Maackia amurensis agglutinin and Sambucus nigra agglutinin). On the uncapacitated sperm surface, sialylglycoconjugates were found be localized from the post-acrosomal region of the sperm head to the tail middle piece, whereas after invitro capacitation these molecules were only found in a small area of the post-acrosomal region. The surface of capacitated human spermatozoa was also investigated by specifically radiolabelling its terminal sialic acid residues. A 20 kDa glycoprotein, which was partially purified by anion-exchange chromatography, was the main component of the sialylglycoconjugate pattern after in-vitro capacitation.Keywords
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