Multiple isotypes of α‐ and β‐tubulin in the plant Phaseolus vulgaris

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Abstract
The tubulins of the plant Phaseolus vulgaris have been identified in cell extracts by a combination of western blotting of 1D and 2D gels and detection by monoclonal antibodies specific for α‐ and β‐tubulin. Plant α‐tubulin differs from mammalian tubulin in that it migrates ahead of the β‐subunit on Laemmli SDS gels. The Phaseolus root tip extracts contain 4 α‐ and 4 β‐tubulin isotypes which are electrophoretically separable on 2D gels under a number of preparative conditions. The results indicate a complex expression of multiple tubulin isotypes in plant tissues.