Infection of Rats with Bovine Leukaemia Virus: Establishment of a Virus-producing Rat Cell Line
- 1 July 1989
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 70 (7) , 1929-1932
- https://doi.org/10.1099/0022-1317-70-7-1929
Abstract
Adult rats were infected with bovine leukemia virus (BLV). Inoculated rats persistently produced antibodies directed against viral structural proteins. No major pathogenesis in infected rats was found during 2 years of observation. It was possible to recover the virus from rat spleen several months after infection. A cell line, R(BLV), was established from rat spleen; this contained integrated BLV provirus. R(BLV) cells kept for over 80 passages in vitro produced viral particles with the properties of BLV. Provirus reintegration and/or amplification occurred in R(BLV) cells. The cell line was found to be tumorigenic in rats, and the virus produced was immunogenic. R(BLV) cells represent the first described BLV-producing rat cell line. Proven persistent infection with BLV indirectly suggests that rats can serve as a reservoir of BLV in nature.This publication has 9 references indexed in Scilit:
- BOVINE LEUKEMIA-VIRUS - ISOLATION AND CHARACTERIZATION OF NONPRODUCER CELL CLONES1987
- ISOLATION AND CHARACTERIZATION OF CELL CLONES PRODUCING VARIOUS AMOUNTS OF BOVINE LEUKOSIS VIRUS1985
- Genomes of evolutionarily divergent members of the human T-cell leukemia virus family (HTLV-I and HTLV-II) are highly conserved, especially in pX.Proceedings of the National Academy of Sciences, 1984
- Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virusProceedings of the National Academy of Sciences, 1982
- Experiments with cloned complete tumor-derived bovine leukemia virus information prove that the virus is totally exogenous to its target animal speciesJournal of Virology, 1981
- Goat Lymphosarcoma From Bovine Leukemia Virus23JNCI Journal of the National Cancer Institute, 1981
- A Rapid Neutralization Test for Antibodies to Bovine Leukemia Virus, With the Use of Rhabdovirus PseudotypesJNCI Journal of the National Cancer Institute, 1979
- DEVELOPMENT OF AN INVITRO INFECTIVITY ASSAY FOR C-TYPE BOVINE LEUKEMIA-VIRUS1976
- INVITRO TRANSMISSION AND PROPAGATION OF BOVINE LEUKEMIA-VIRUS IN MONOLAYER CELL-CULTURES1976