Effects of synthesis inhibition and nervous activity on concentrations of neuronal substance P

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Abstract
When dorsal root ganglia were incubated in vitro with a range of concentrations of anisomycin, incorporation of [3H]lysine into protein and synthesis of substance P (SP) were inhibited to a similar extent confirming that SP is synthesised by a conventional ribosomal mechanism. Doses of anisomycin sufficient to inhibit protein synthesis in mouse CNS in vivo by greater than 95% did not cause a significant fall in the SP content of any of five brain areas, spinal cord, dorsal root ganglia or cornea over an 8 h period. Electrical stimulation of the hind limbs however, produced a 25% fall in the SP content of the dorsal spinal cord of these animals. These findings suggest that the turnover-time of neuronal SP is much longer than that of non-peptide neurotransmitters.