Metabolism of cyclic ADP-ribose in opossum kidney renal epithelial cells
- 1 March 1995
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 268 (3) , C741-C746
- https://doi.org/10.1152/ajpcell.1995.268.3.c741
Abstract
We have previously shown that NAD+ inhibits renal Na(+)-Pi symport; however, the biochemical mechanism of NAD+ in this action is not clarified. We now propose that NAD+ acts indirectly by first being converted to cyclic ADP-ribose (cADPR), a potent stimulator of intracellular Ca2+ mobilization. In permeabilized opossum kidney (OK) cells, a cell line often employed as a model for study of proximal tubular epithelial transport, cADPR is synthesized from beta-NAD+ in a substrate concentration (0.01-1 mM) and time-dependent manner. That cADPR was generated from beta-NAD+ by OK cells was verified by coelution with authentic cADPR on anion exchange high-performance liquid chromatography and by homologous desensitization of the Ca2+ release bioassay to authentic cADPR. cADPR synthesized by permeabilized OK cells was not influenced by the addition of parathyroid hormone. The OK cell also contains the enzyme activity necessary to catalyze catabolism of cADPR. Identification of these two key enzyme activities of cADPR metabolism in OK cells is consistent with a possible role of cADPR in regulation of the Na(+)-Pi symporter by NAD+ in response to metabolic stimuli. dKeywords
This publication has 12 references indexed in Scilit:
- Expression of Na/Pi cotransport from opossum kidney cells in Xenopus laevis oocytesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1993
- Calcium Mobilization by Dual Receptors During Fertilization of Sea Urchin EggsScience, 1993
- Ryanodine receptors: how many, where and why?Trends in Pharmacological Sciences, 1993
- Cyclic ADP-Ribose in Insulin Secretion from Pancreatic βCellsScience, 1993
- Expression of a Ryanodine Receptor-Ca 2+ Channel that is Regulated by TGF-βScience, 1992
- Ca 2+ -Induced Ca 2+ Release in Sea Urchin Egg Homogenates: Modulation by Cyclic ADP-RiboseScience, 1991
- ADP-ribosyl cyclase: an enzyme that cyclizes NAD+ into a calcium-mobilizing metabolite.Cell Regulation, 1991
- Parathyroid hormone inhibition of Na+/phosphate cotransport in OK cells: intracellular [Ca2+] as a second messengerBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1989
- Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cellsAnalytical Biochemistry, 1985
- Possible role of nicotinamide adenine dinucleotide as an intracellular regulator of renal transport of phosphate in the rat.Journal of Clinical Investigation, 1981