RNA in Defense: CRISPRs Protect Prokaryotes against Mobile Genetic Elements
Open Access
- 2 February 2011
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Perspectives in Biology
- Vol. 4 (6) , a003657
- https://doi.org/10.1101/cshperspect.a003657
Abstract
The CRISPR/Cas system in prokaryotes provides resistance against invading viruses and plasmids. Three distinct stages in the mechanism can be recognized. Initially, fragments of invader DNA are integrated as new spacers into the repetitive CRISPR locus. Subsequently, the CRISPR is transcribed and the transcript is cleaved by a Cas protein within the repeats, generating short RNAs (crRNAs) that contain the spacer sequence. Finally, crRNAs guide the Cas protein machinery to a complementary invader target, either DNA or RNA, resulting in inhibition of virus or plasmid proliferation. In this article, we discuss our current understanding of this fascinating adaptive and heritable defense system, and describe functional similarities and differences with RNAi in eukaryotes.Keywords
This publication has 65 references indexed in Scilit:
- Understanding the Differences between Genome Sequences of Escherichia coli B Strains REL606 and BL21(DE3) and Comparison of the E. coli B and K-12 GenomesJournal of Molecular Biology, 2009
- CRISPR-based adaptive and heritable immunity in prokaryotesTrends in Biochemical Sciences, 2009
- Analysis of CRISPR in Streptococcus mutans suggests frequent occurrence of acquired immunity against infection by M102-like bacteriophagesMicrobiology, 2009
- X‐ray crystal structure of a CRISPR‐associated protein, Cse2, from Thermus thermophilus HB8Proteins-Structure Function and Bioinformatics, 2008
- Phage Response to CRISPR-Encoded Resistance in Streptococcus thermophilusJournal of Bacteriology, 2008
- Structural Basis for Double-Stranded RNA Processing by DicerScience, 2006
- Identification of novel non‐coding RNAs as potential antisense regulators in the archaeon Sulfolobus solfataricusMolecular Microbiology, 2004
- Identification of genes that are associated with DNA repeats in prokaryotesMolecular Microbiology, 2002
- A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysisNucleic Acids Research, 2002
- High abundance of viruses found in aquatic environmentsNature, 1989