Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker
- 1 June 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (6) , 2849-52
- https://doi.org/10.1128/aem.67.6.2849-2852.2001
Abstract
For diagnosis of citrus bacterial canker by PCR, an internal standard is employed to ensure the quality of the DNA extraction and that proper requisites exist for the amplification reaction. The ratio of PCR products from the internal standard and bacterial target is used to estimate the initial bacterial concentration in citrus tissues with lesions.Keywords
This publication has 11 references indexed in Scilit:
- A simple extraction procedure for efficient routine detection of pathogenic bacteria in plant material by polymerase chain reactionJournal of Microbiological Methods, 1999
- A simple and efficient PCR method for the detection ofAgrobacterium tumefaciensin plant tumoursJournal of Applied Microbiology, 1999
- The construction and use of a PCR internal controlMolecular and Cellular Probes, 1998
- Rapid and Sensitive Colorimetric Detection of Xanthomonas axonopodis pv. citri by Immunocapture and a Nested-Polymerase Chain Reaction AssayPhytopathology®, 1996
- The Polymerase Chain Reaction and Plant Disease DiagnosisAnnual Review of Phytopathology, 1993
- Population Dynamics of Strains ofXanthomonas campestrisDiffering in Aggressiveness on Swingle Citrumelo and GrapefruitPhytopathology®, 1991
- Variation in Aggressiveness ofXanthomonas campestrispv.citrumeloAssociated with Citrus Bacterial Spot in Florida Citrus NurseriesPhytopathology®, 1990
- Cultivar-Specific Interactions for Strains of Xanthomonas campestris from Florida that Cause Citrus Canker and Citrus Bacterial SpotPlant Disease, 1990
- Survival of Xanthomonas campestris pv. citri in Citrus Plant Debris and Soil in Florida and ArgentinaPlant Disease, 1987
- Canker, a Threat to Citrus in the Gulf-Coast StatesPlant Disease, 1983