Early expression of a β1‐adrenergic receptor and catecholamines in Xenopus oocytes and embryos
Open Access
- 10 November 1997
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 417 (2) , 184-190
- https://doi.org/10.1016/s0014-5793(97)01278-7
Abstract
From a Xenopus stage 11 cDNA library, we have cloned a gene, termed X-β 1AR, whose sequence is highly homologous to that of the human β1-adrenergic receptor. As shown by RT-PCR assay, X-β 1AR RNA is present in the mature oocyte, decreases after fertilization up to stage 6 and then gradually increases during gastrulation. Binding studies performed with radiolabeled ligands reveal that X-β 1AR RNA is translated into the receptor protein. Furthermore, noradrenaline and adrenaline are also detected in oocytes and early embryos. The concomitant presence of β1-adrenergic receptors and catecholamines suggest that this ligand-receptor couple could play a role in the very early stages of embryonic development.Keywords
This publication has 38 references indexed in Scilit:
- smoothened encodes a receptor-like serpentine protein required for hedgehog signallingNature, 1996
- A new member of the frizzled family from Drosophila functions as a Wingless receptorNature, 1996
- GTP-binding proteins and early embryogenesis in XenopusCellular Signalling, 1995
- STRUCTURE AND FUNCTION OF G PROTEIN-COUPLED RECEPTORSAnnual Review of Biochemistry, 1994
- Xenopus laevis oocyte Gα subunits mRNAs Detection and quantitation during oogenesis and early embryogenesis by competitive reverse PCRFEBS Letters, 1992
- Expression cloning of an activin receptor, a predicted transmembrane serine kinaseCell, 1991
- Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structuresCell, 1990
- Selective Amplification And Cloning Of Four New Members Of The G Protein-coupled Receptor FamilyScience, 1989
- Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsinNature, 1986
- A major developmental transition in early xenopus embryos: I. characterization and timing of cellular changes at the midblastula stageCell, 1982