Attempts to Find Human Adenovirus Type-12 Tumor Antigens in Human Tumors2

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Abstract
Complement-fixation (CF) tests were used in the search for adenovirus type-12 (A-12) tumor antigens in human tumors. Approximately 25% of 112 human tumor and nontumor tissues reacted in CF tests with sera from hamsters bearing transplanted A-12 tumors. Not all pools of sera from A-12 tumor-bearing hamsters reacted with all positive human tissues. However, 8 reactions, apparently specific for A-12 tumor antigens, were found. Since these hamster sera, as a result of tumor transplantation, could contain hamster isoantibodies cross-reacting with human tissues, such antibodies were sought with the use of the hamster Fortner Sarcoma #3B (F Sa) as antigen. Reactivity with F Sa antigen(s) was found in 15% of sera from individual A-12 tumor-bearing or normal hamsters. Two serum pools from A-12 tumorous hamsters, reactive with human tumor tissue, also had anti-F Sa activity. Pools of hamster A-12 tumor sera lacking F Sa reactivity failed to react with the “positive” human tumors. Autochthonous human tumor and serum combinations were negative for tumor-specific antigens by the CF technique.