Ultrastructural studies on exogenously dormant ascospores of Daldinia concentrica

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Abstract
Exogenously dormant ascospores of Daldinia concentrica have been studied using light and electron microscope techniques. Tests have shown that ascospores are rendered non-viable after 10 min treatment with fixative. Thin sections and shadowed, chemically cleaned preparations revealed a multilayered ascospore wall, parts of which are differentiated to form a longitudinal germ fissure. A microfibrillar component is associated with the germ fissure, and enzymic digestion indicates that these fibrils could be chitin. It is also suggested that sporopollenin may be present in certain wall layers.