Characterization of an N6-oxopropenyl-′-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospry ionization tandem mass spectrometry
Open Access
- 1 January 1996
- journal article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 17 (5) , 1167-1170
- https://doi.org/10.1093/carcin/17.5.1167
Abstract
Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct M1G-dR has been detected in rat and human liver. Random mutagenesis studies with MDA-modified DNA and recent 32P-postlabeling studies indicate that in addition to M1G-dR, adducts to deoxyadenosine may also be formed. We have utilized liquid chromatography coupled with electrospray ionization tandem mass spectrometry to characterize an N6-oxopropenyl-2′-deoxyadenosine adduct (M1A-dR) in calfthymus DNA modified with MDA.Keywords
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