Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum
- 1 April 1999
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 55 (4) , 679-682
- https://doi.org/10.1007/s000180050324
Abstract
A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.Keywords
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