A bilayer cell‐free protein synthesis system for high‐throughput screening of gene products
Open Access
- 1 February 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 514 (1) , 102-105
- https://doi.org/10.1016/s0014-5793(02)02329-3
Abstract
A high‐throughput cell‐free protein synthesis method has been described. The methodology is based on a bilayer diffusion system that enables the continuous supply of substrates, together with the con...Keywords
This publication has 12 references indexed in Scilit:
- A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomesProceedings of the National Academy of Sciences, 2000
- A new class of enzyme acting on damaged ribosomes: ribosomal RNA apurinic site specific lyase found in wheat germThe EMBO Journal, 1999
- Rate of Translation of Natural mRNAs in an Optimizedin VitroSystemArchives of Biochemistry and Biophysics, 1996
- Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826Journal of Fermentation and Bioengineering, 1993
- Production of an enzymatic active protein using a continuous flow cell-free translation systemJournal of Biotechnology, 1992
- A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High YieldScience, 1988
- Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-ANature, 1986
- Translational accuracy in vitroCell, 1982
- Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat GermProceedings of the National Academy of Sciences, 1973