Alginate encapsulation of genetically engineered mammalian cells: comparison of production devices, methods and microcapsule characteristics

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Abstract
Primary objective: Microencapsulation is a novel method for in vivo immunoprotection of genetically engineered mammalian cells. This study aimed at optimizing alginate/poly-L-lysine/alginate (APA) microencapsulation of mammalian cells in small size (Methods and procedures: Alginate preparations with higher or lower viscosity were used with three different methods: (i) vibrating nozzle, (ii) coaxial gas flow extrusion (AirJet) and (iii) laminar jet break-up (JetCutter). Parameters and device settings for the production of microcapsules with specific characteristics were defined for all three methods. Mechanical stability of APA microcapsules and cell viability of encapsulated cells were investigated in long-term culture and in an animal model. Main results: Uniform spherical beads with a mean diameter Conclusions: The described methods and devices are able to produce APA microcapsules of small size and uniform shape which are mechanically stable in culture and may maintain the viability of the enclosed cells over extended periods of time. These microcapsules seem to be suitable for further therapeutic studies in an animal model of human disease.

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