Further Considerations on a Simple Histological, Method for Identification of Osteoid Matrix
- 1 January 1974
- journal article
- Published by Taylor & Francis in Stain Technology
- Vol. 49 (6) , 367-373
- https://doi.org/10.3109/10520297409117013
Abstract
The authors have proposed fixation in cyanuric chloride as a means of inducing marked differences in acidophilia between mineralized bone matrix and osteoid. The effects of cyanuric chloride fixation are investigated in more detail here. Dye-extinction tests performed on sections from decalcified rachitic rat calvariae fixed with cyanuric chloride demonstrated a much higher isoelectric point in osteoid matrix than in mineralized matrix. Cyanuric chloride fixation resulted in high isoelectric points for fixed un mineralized proteins in general. Micro radiography revealed no decalcification in bone during fixation in cyanuric chloride.Keywords
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