A procedure for data analysis of the rodent micronucleus test involving a historical control

1 January 1989

journal article
research article
Published by Wiley in Environmental and Molecular Mutagenesis

Vol. 13 (4) , 347-356
https://doi.org/10.1002/em.2850130412

Abstract

No standard procedure of data analysis for rodent micronucleus tests involving historical controls has been established. In the present paper, under the presumption that the distribution of the historical control is stable and reliable, a procedure with three statistical steps is proposed to analyze the frequency of micronucleated polychromatic erythrocytes (MNPCEs). In the first step, the frequencies of MNPCEs in negative and positive control groups of a current experiment of the micronucleus test are compared with the distribution of historical negative and positive controls to examine the technical validity of the current experiment. In the second step, the frequency of MNPCEs in each treatment group is compared with the distribution of the historical negative control. In the third step, the dose‐response relation is tested with the Cochran‐Armitage trend test. A Monte Carlo simulation study shows that the power of this procedure is acceptable and also this procedure is robust. An application of this procedure on real data reveals that it is effective in detecting clastogenic chemicals when the probability of a type I error is nearly .01.

Keywords

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