MECHANISM OF ACTION OF THE LACTIC DEHYDROGENASE OF THE MAMMALIAN ERYTHROCYTE: II. THE MECHANISM OF SUBSTRATE INHIBITION

Abstract
The inhibition of lactic dehydrogenase produced by an excess of the substrate pyruvate has been shown to be of noncompetitive type. Thus, two sites on the enzyme appear to be involved: one for the activation of pyruvate, and a second one which, when complexed with a molecule of pyruvate, renders the enzyme inactive.