A Chemical and Histological Study of Artificial Caries in Human Dental Enamel in vitro

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Abstract
A method is described for the preparation of caries-like lesions in human enamel using a short column of methyl cellulose gel to restrict demineralisation by lactate buffer. The procedure permits the analytical determination of the ions leaving the enamel. It is found that there is a preferential removal of calcium compared to phosphorus and this is interpreted in terms of a calcium-deficient hydroxyapatite system. The lesions produced show the apparently intact surface zone characteristic of the early carious lesion. Ground sections, when examined by polarised light, show a surface zone with 1–5% pore volume over a more extensively demineralised main body of the lesion. Only the dark zone was missing in sections examined after imbibition with quinoline.

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