A Streamlined Method of Subfragment One Preparation from Myosin1
- 1 October 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 98 (4) , 1143-1145
- https://doi.org/10.1093/oxfordjournals.jbchem.a135365
Abstract
A rapid procedure for isolating subfragment one (SF1) from myosin was found. SF1 can be isolated specifically from proteolytic digests of myosin in the presence of a millimolar concentration of magnesium chloride. Under such ionic conditions all of the rod portion and undigested myosin is selectively precipitated. A nucleotide trapping experiment indicated how important quick preparation of SF1 is for maintaining the active site structure. This method can also be utilized in the preparation of heavy meromyosin.This publication has 5 references indexed in Scilit:
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