TUMOR-ASSOCIATED ANTIGENS ON BOVINE LEUKEMIA VIRUS-INDUCED BOVINE LYMPHOSARCOMA IDENTIFIED BY MONOCLONAL-ANTIBODIES

  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (3) , 1174-1180
Abstract
Thirteen monoclonal antibodies directed against tumor cells from cattle from enzootic bovine leukosis (EBL) were obtained. They reacted with tumor cells but not with normal bovine cells or bovine leukemia virus antigens. According to the reactivities of these antibodies with 19 individual tumors, the 13 monoclonal antibodies can be divided into 3 groups. Antibodies of the 1st group reacted with all the EBL tested; those of the 2nd group reacted with several, but not all, of the EBL tested; and those of the 3rd group reacted only with homologous tumor cells. Therefore, tumor-associated antigen (TAA) on the EBL tumor may possess common TAA, partially common TAA and individually distinct TAA. The TAA were solubilized from the tumor cells by treatment with 0.2% sodium deoxycholate and partially purified by diethylaminoethyl-cellulose column chromatography. Eleven of the 13 monoclonal antibodies reacted with this soluble TAA. The monoclonal antibodies belonging to the 1st group inhibited in vitro the growth of the bovine lymphoid cell line derived from the EBL tumor. [The use of these monoclonal antibodies to diagnose EBL was described.].