Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca 2+ Transients in Ciliated Tracheal Epithelial Cells
Open Access
- 1 December 2003
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (12) , 7208-7210
- https://doi.org/10.1128/iai.71.12.7208-7210.2003
Abstract
To study initial Bordetella bronchiseptica -tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca 2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca 2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca 2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica -host cell interactions that can contribute to pathogenicity or to host cell defense.Keywords
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