Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation
Open Access
- 1 October 2007
- journal article
- Published by Wiley
- Vol. 581 (26) , 5060-5064
- https://doi.org/10.1016/j.febslet.2007.09.048
Abstract
The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5 Å resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid‐opening mechanism.Keywords
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