AN ISOTOPIC MICROMETHOD FOR THE MEASUREMENT OF CHOLINESTERASE ACTIVITY IN INDIVIDUAL CELLS
- 1 November 1969
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 16 (11) , 1523-1528
- https://doi.org/10.1111/j.1471-4159.1969.tb09907.x
Abstract
—A microisotopic method for measuring acetylcholinesterase activity in isolated cells is described. The assay employs [14C]acetylcholine and can measure 7 × 10‐12 moles of acetylcholine hydrolysed/hr in 50‐150 samples per experiment.The method described has been applied to the measurement of cholinesterase activity in individual sympathetic ganglion cells of the cat. It has been shown that under standard conditions the substrate has complete access to the enzymatic site.Keywords
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