INVITRO INFECTION OF LYMPHOCYTES WITH MAREKS-DISEASE VIRUS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 69  (3) , 709-713
Abstract
Suspension cultures of chicken splenic lymphocytes, incubated at 41.degree. C, became infected with Marek''s disease virus (MDV) following exposure to other infected lymphocytes infected chicken kidney monolayer cultures or cell-free MDV. Both viral antigen expression and virus isolation could be demonstrated after > 40 passages made by the addition of fresh spleen cells at 2-3 day intervals. Susceptibility of spleen cells from bursectomized chickens was markedly lower than that of cells from intact birds. When spleen cell suspensions were depleted of cells having characteristics of bursa-derived cells, e.g., those with surface IgM, Fc receptors or ability to adhere to nylon wool, the susceptibility of the cell suspension was diminished. Enrichment of the suspension with cells having those features enhanced overall susceptibility. The target cells for virus infection in vitro also were shown to be nonphagocytic, to be of low or medium density and to bear Ia-like antigen. In vitro susceptibility to infection of spleen cells did not correlate with the genetic susceptibility of the donor to Marek''s disease.