Amino acid sequence determination of guuanyl‐specific ribonuclease Sa from Streptomyces aureofaciens
- 15 December 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 209 (2) , 335-339
- https://doi.org/10.1016/0014-5793(86)81138-3
Abstract
Using automated Edman degradation of two nonfractionated peptide mixtures of tryptic and staphylococcal protease digests of the protein, the complete amino acid sequence of the guanyl‐specific ribonuclease Sa from Streptomyces aureofaciens was established. Ribonuclease Sa contains 96 amino acid residues (M r 10 566). A 50% sequence homology of ribonuclease Sa to the guanyl‐specific ribonuclease St from S. erythrew was found.Keywords
This publication has 10 references indexed in Scilit:
- Express analysis of protein amino acid sequencesFEBS Letters, 1986
- Amino acid sequence and S‐S bonds of Penicillium brevicompactum guanyl‐specific ribonucleaseFEBS Letters, 1984
- Use of fluorescamine as an effective blocking reagent to reduce the background in protein sequence analyses by the beckman automated sequencerAnalytical Biochemistry, 1981
- Automated amino acid sequence of small peptides utilizing PolybreneAnalytical Biochemistry, 1978
- The amino acid sequence of ribonuclease StFEBS Letters, 1976
- Advances in the gas chromatographic analysis of amino acid phenyl- and methylthiohydantoinsAnalytical Biochemistry, 1972
- Exocellular ribonuclease from Streptomyces aureofaciens II. Properties and specificityBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- Exocellular ribonuclease from Streptomyces aureofaciens I. Isolation and purificationBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- A Protein SequenatorEuropean Journal of Biochemistry, 1967
- The Preparation and Enzymatic Hydrolysis of Reduced and S-Carboxymethylated ProteinsJournal of Biological Chemistry, 1963