A novel type A2 neurotoxin gene cluster inClostridium botulinumstrain Mascarpone
Open Access
- 1 August 2006
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 261 (1) , 88-94
- https://doi.org/10.1111/j.1574-6968.2006.00331.x
Abstract
The partial nucleotide sequence (∼10kb) of the cluster of genes encoding the botulinum neurotoxin complex in Clostridium botulinum type A strain Mascarpone was determined. The analysis revealed six ORFs (orfs), which were organized as in the type A2 and type A3 botulinum neurotoxin gene clusters of strains Kyoto-F and NCTC 2916, respectively. While the orfs at the proximal and distal ends of the sequence (orfX2 and bont/A genes) shared a high level of similarity with the corresponding sequences of strain Kyoto-F, the segment encompassing the orfX1 and botR/A genes within the sequence exhibited a higher degree of homology to the related region in strain NCTC 2916. The mosaic structure of the Mascarpone neurotoxin gene cluster suggests recombinational exchanges.Keywords
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