RELATION OF THE BUTANOL-SOLUBILIZED RABBIT HISTOCOMPATIBILITY ANTIGENS TO THE SUPPRESSION OF ANTIBODY-PRODUCING ALLOGEN-EIC LYMPH NODE CELLS

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Abstract
Soluble antigen prepared by Triton and butanol from cell membrane fragments of rabbit lymph node and spleen is associated with the suppression or rejection of allogeneic lymph node cells by its abilities to inhibit or absorb the supres -sive antibody in rabbit antileucocyte serum, to induce actively in normal rabbits the rejection of transferred lymph node cells and to stimulate production of antibodies suppressive for allogeneic lymph node cells. This can be demonstrated in cell transfer or, in vitro, in the hemolytic antibody plaque test. Analysis by chromatography and sucrose density gradient ultracentrlfugation shows that the rejection-associated antigen can be found within approximately 1/2 the total protein of the butanol extract, and suggests nonidentity of the antigen detectable by inhibition of suppressive antibody and that detectable serologically. Further evidence of nonidentity of the antigens and antibodies detected is given by substantial disproportlonallty of titers determined in suppressive and serological tests in primary and secondary antisera to whole allogeneic cells, respectively, and in secondary tertiary anti-sera to the butanol-solubilized antigen.

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