Processing of major histocompatibility class I-restricted antigens in the endoplasmic reticulum.
Open Access
- 1 April 1995
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 181 (4) , 1481-1491
- https://doi.org/10.1084/jem.181.4.1481
Abstract
We have introduced long precursor peptides directly into the endoplasmic reticulum (ER) of a mutant cell line (T2-Db) that lacks the ability to transport peptides from the cytosol to the ER in a transporter associated with antigen processing (TAP) dependent way. This was done by expressing various influenza A-derived peptides containing the naturally processed epitope ASNENMDAM (366-374) preceded by the influenza hemagglutinin ER translocation sequence. Peptides derived from these minigenes that became associated with Db were isolated and identified by combined reversed phase liquid chromatography and detection by cytotoxic T lymphocytes. Our results establish that NH2-terminal extensions of at least 40 residues can be trimmed from peptides entering the ER, but that proteolysis of larger proteins may be limited.Keywords
This publication has 53 references indexed in Scilit:
- Peptide selection by class I molecules of the major histocompatibility complexCurrent Biology, 1993
- Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cellsNature, 1993
- HLA-A2 molecules in an antigen-processing mutant cell contain signal sequence-derived peptidesNature, 1992
- Transport Protein Genes in the Murine MHC: Possible Implications for Antigen ProcessingScience, 1990
- Sequences encoded in the class II region of the MHC related to the 'ABC' superfamily of transportersNature, 1990
- MHC class II region encoding proteins related to the muKidrug resistance family of transmembrane transportersNature, 1990
- Isolation and analysis of naturally processed viral peptides as recognized by cytotoxic T cellsNature, 1990
- Isolation of an endogenously processed immunodominant viral peptide from the class I H–2Kb moleculeNature, 1990
- The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells.The Journal of cell biology, 1989
- The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptidesCell, 1986