Preparation and Characterization of Inverted Cell Envelopes of Halobacterium halobium
- 1 October 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 111 (2) , 411-418
- https://doi.org/10.1111/j.1432-1033.1980.tb04955.x
Abstract
Inside-out cell envelopes from H. halobium R1M1 were isolated by sonication and subsequent sucrose-density-gradient fractionation. The inverted cell envelopes transport protons from the exterior of the vesicles by a light-dependent, uncoupler-inhibited process. Enzymes usually facing the inside of the cell are exposed to the external medium in this preparation.This publication has 13 references indexed in Scilit:
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