EXCRETION OF URINARY 17-KETOSTEROIDS BY THE MALE RABBIT

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Abstract
An anhydrous Zimmerman reaction for assay of 17-ketosteroids was applied to a partially purified neutral ketonic fraction of rabbit urine. Precision studies, recovery expts., and chromogen purity studies indicate that the method is sufficiently accurate to warrant application utilizing the rabbit. The mean excretion of 17-ketosteroids in the normal c? rabbit is 2.23 mg./48 hrs. as indicated by assay of 109 urines collected from 18 animals. The excretion of 17-ketosteroids was relatively constant despite variations in urine vol./48 hrs. Two animals injected with Brown-Pearce tumor suspension excreted greatly reduced amts. of 17-ketosteroids. This is correlated with tumor cell metastases in the adrenal cortical gland. Methyl-cholan-threne deposited in the adrenals of 4 animals for 300 days induced histiologic changes in the adrenal glands. Three of these animals excreted significantly lower amts. of 17-ketosteroids. Depression of 17-ketosteroid excretion is thought to be referable to the induced histologic changes in the adrenal cortical gland. Such evidence supports the adrenal source hypothesis of 17-ketosteroids.

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