Excystation and Cultivation In vitro and In ovo of Cyathocotyle bushiensis (Trematoda) Metacercariae

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Abstract
Encysted metacercariae of Cyathocotyle bushiensis excysted in an alkaline bile-trypsin medium in the absence of acid-pepsin pretreatment. Cysts pretreated in acid-pepsin for more than 2 hr did not excyst. The percentage of cysts that excysted in alkaline bile-trypsin after storage in Locke''s 1:1 at 4 C was 94% at 1 wk, 62% at 4 wk, and 15% at 8 wk. Domestic chicks could not be infected with cysts, but excysted metacercariae developed and grew on the chick chorioallantois. Excysted metacercariae cultivated in NCTC 135 survived about 8 days but did not develop; those cultivated in NCTC 135 plus 20% hen''s egg yolk doubled their body area and produced abnormal eggs in 3 days; others cultivated in NCTC 135 plus 50% chick serum increased their body area about 10.times. and became ovigerous by day 8.