Nucleolar Stain and Nucleal Reaction
- 1 June 1943
- journal article
- research article
- Published by University of Chicago Press in Botanical Gazette
- Vol. 104 (4) , 645-649
- https://doi.org/10.1086/335179
Abstract
In the Feulgen and Light Green staining technique of Semmens and Bhaduri, the use of an alkali (Na meta-bisulphate) as a mordant for the nucleoli makes all the difference between an ordinary counterstain, as used by others, and a specific stain for nucleoli. Various chemical aspects of this staining technique are entered into and difficulties are pointed out, with their chemical reasons. The method is essentially (1) the Feulgen stain (without acetic acid fixation), (2) the alkali mordant for the nucleoli, (3) the use of a green dye as a specific stain for nucleolin.This publication has 5 references indexed in Scilit:
- Cytological analysis of structural hybridity inRhoeo discolor hanceJournal of Genetics, 1942
- CHROMOSOME CHEMISTRY AND GENE ACTIONNature, 1941
- Ribonucleic Acids in Both Nucleus and Cytoplasm, and the Function of the NucleolusProceedings of the National Academy of Sciences, 1940
- Use of the Feulgen Reaction in Cytology I. Effect of Fixatives on the ReactionBotanical Gazette, 1939
- Studies in the microchemistry of the cell. I.—The chromatin content of normal and malignant cells, as demonstrated by Feulgen's “Nuclealreaktion."Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1928