Comparison of Three Methods for Recovery of Campylobacter spp. from Broiler Carcasses

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Abstract
We compared three enrichment methods, with different sampling times (mid-and endpoint incubation) and various dilutions of enrichment culture for productivity in the isolation of Campylobacter spp. from 50 retail-level chicken carcasses. We subcultured the enrichment cultures onto three Campylobacter spp.-selective media (Campy-BAP, CCDA, Campy-Cefex) to compare yields of the organism. The highest yield (43/50) of Campylobacter spp. from these carcasses was derived by using the 24-h enrichment culture of Hunt & Radle diluted 1:100 before plating onto any of the three selective plating media. When all carcass analyses were combined, Campylobacter spp. was recovered from 49 of 50 broilers. This study indicates the optimum approaches for the recovery of Campylobacter spp., as well as the high incidence of the organism among the broiler carcasses tested.

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