Evidence for lipoxin formation by bovine polymorphonuclear leukocytes via triple dioxygenation of arachidonic acid
- 8 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 228 (1) , 167-171
- https://doi.org/10.1016/0014-5793(88)80609-4
Abstract
Incubation of bovine polymorphonuclear leukocytes (PMNs) with arachidonic acid leads to the formation of four lipoxins. The same lipoxins are also formed upon incubation of bovine PMNs with 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, 5-hydroxy-6-trans-8,11,14-cis-eicosatetraenoic acid, 5(S)-hydroperoxy, 15(S)-hydroxy-6,13-trans-8, 11-cis-eicosatetraenoic acid or 5(S), 15(S)-dihydroxy-6,13-trans-8,11-cis-eicosatetraenoic acid. A 5,6-epoxide as intermediate in lipoxin formation in the bovine PMN is highly improbable because the 5-hydroxy compounds are as good substrates as the 5-hydroperoxy compounds. Moreover, the two main lipoxins were found to coelute with the two lipoxins produced via a triple dioxygenation of arachidonic acid by soybean lipoxygenase-1. Hence the bovine PMN is the first cell for which evidence is presented that the formation of lipoxins proceeds mainly via triple dioxygenation and not via 15-hydroxy-leukotriene A4 as is proposed for human and porcine PMNS.Keywords
This publication has 16 references indexed in Scilit:
- Trihydroxytetraenes: A novel series of compounds formed from arachidonic acid in human leukocytesPublished by Elsevier ,2004
- A phospholipase A2 isoenzyme provokes lipoxin B formation from endogenous sources of arachidonic acid in porcine leukocytesBiochemical and Biophysical Research Communications, 1987
- Rapid, simple and efficient extraction of arachidonic acid metabolites, including the sulphidopeptide leukotrienes LTC4 and LTD4, using octadecyl reversed-phase extraction columnsJournal of Chromatography B: Biomedical Sciences and Applications, 1986
- Evidence for a 5(6)‐epoxytetraene intermediate in the biosynthesis of lipoxins in human leukocytesFEBS Letters, 1986
- The lipoxins: Determination of their biosynthesisPharmacological Research Communications, 1986
- On the biosynthesis and the structure of lipoxin BTetrahedron Letters, 1985
- Leukotriene formation by bovine polymorphonuclear leukocytesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Separation and quantitation of leukotrienes by reversed-phase high-performance liquid chromatographyProstaglandins, Leukotrienes and Medicine, 1984
- Rapid extraction of leukotrienes from biologic fluids and quantitation by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1982
- Double dioxygenation of arachidonic acid by soybean lipoxygenase-1 kinetics and regio-stereo specificities of the reaction stepsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981