Production of cytoplasmic polyhedrosis virus (CPV) polyhedra in a gamma irradiatedLymantria dispar cell line

Publisher Website
Google Scholar
Abstract
Lymantria dispar cells were exposed to different doses of gamma radiation one hour after infection with cytoplasmic polyhedrosis virus (CPV). It was found that irradiated cells can produce infectious polyhedra. Modifications in the structure and in the process of maturation of the polyhedra were noted. The number of polyhedra per cell increased significantly after cell irradiation at 104 and 105 rads but no change was noted after cell treatment at 102 rads. On the other hand, morphological changes and a high mortality rate were noted in cell cultures treated at intensities higher than 103 rads. Therefore, the total yield of polyhedra produced when using 102 or 104 rads was similar to that obtained in normal cells but dropped significantly after cell irradiation at 105 rads.