Retroviral vector sequences inhibit human β-globin gene expression in transgenic mice
Open Access
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (21) , 4477-4481
- https://doi.org/10.1093/nar/22.21.4477
Abstract
The DNase I hypersensitive site 5′ HS2 of the human β-globin locus control region confers positionindependent, high-level expression on the human β- globin gene in transgenic mice. When a 5′ HS2 β-globin construct is flanked by retroviral vector sequences derived from Moloney Murine Leukemia Virus (MoMLV), expression of the β-globin gene is severely inhibited. Apparently, one or more elements within the MoMLV genome is capable of repressing transcription of the human β-globin gene in transgenic mice. A construct lacking the retroviral enhancer also fails to express the β-globin gene, indicating that this region of the virus is not essential for repression. Further analysis may permit the identification of specific viral sequences that inhibit gene expression; these sequences could then be deleted or mutated to produce improved viral vectors.Keywords
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