DISTRIBUTION OF LEUKOCYTES LABELED WITH IN-111 OXINE IN DOGS WITH ACUTE INFLAMMATORY LESIONS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 21  (11) , 1059-1068
Abstract
The biodistributions of In-111 oxine (with and without leukocyte labeling) of Ga-67 citrate and of In-111 chloride were compared in 30 dogs with chemical and bacterial abscesses and acute joint inflammation. Serial blood samples were taken and tissues radioassayed at 24 h. The concentration of In-111-oxine leukocytes in the 3 types of inflammatory lesion was invariably much higher than that of Ga-67 injected simultaneously. For bacterial abscesses, the mean abscess-to-muscle concentration ratio was 3000 for labeled leukocytes and 72 for Ga-67. Aqueous buffered In-111 oxine sulfate solution appeared better for labeling leukocytes than In-111 oxine in ethanol. When In-111 oxine was not incubated with leukocytes before injection or if the cells were poorly labeled or damaged, the abscess localization was often inferior to that of Ga. Localization of In-111 chloride appeared inferior to that of Ga. No significant difference in distribution in the major organs or inflammatory lesions was demonstrable between labeled suspensions of pure neutrophils harvested by elutriation and mixed cell suspensions of leukocytes after erythrocyte sedimentation with hydroxyethyl starch. For both types of leukocyte suspension labeled with In-111 oxine, the average recovery of cell-bound activity in the circulating blood at 4 h was 32% of the administered activity, inferior to that of P-32 labeled DFP. Apparently, In-111 oxine is a more effective agent than Ga-67 for the detection of acute focal inflammatory lesions if leukocytes are properly labeled; current techniques are unsatisfactory for the study of neutrophil kinetics.