A Technique for the Concentration of Nucleated Bone Marrow Cells for in vitro Manipulation or Cryopreservation Using the IBM 2991 Blood Cell Processor

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Abstract
Bone marrow aspirates (27) were processed on the IBM 2991 Blood Cell Processor to achieve a reduction in volume (> 80%) and in red blood cell contamination (> 75%), without loss (< 20%) of nucleated cells. The procedure was used to concentrate nucleated bone marrow cells either prior to cryopreservation for subsequent autologous transplantation, or prior to incubation with a murine monoclonal antibody (OKT3) for allogeneic transplantation. The procedures used for the concentration, cryopreservation, thawing and infusion do not adversely affect the viability of cells as assessed by in vitro culture (CFU-GM[granulocyte-macrophage progenitor cell]). Of the marrows processed, 12 were reinfused and resulted in successful engraftment.