Purification and Properties of a Basic Endo-1,3-r -Glucanase from Rice (Oryza sativa L.)
Open Access
- 1 July 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 37 (5) , 702-705
- https://doi.org/10.1093/oxfordjournals.pcp.a029002
Abstract
A 1,3-rβ-glucanase purified from rice grain is a 33 kDa monomer with a pI of ≥10.4. The enzyme was determined to be an endo-1,3-rβ-glucanase (EC 3.2.1.39) by end product analysis using Laminaria digitata laminarin as substrate. Its amino-terminal amino acid sequence revealed strong homology to an endo-1,3-rβ-glucanase from barley.Keywords
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