Phagocytosis measured as inhibition of uridine uptake: a method that distinguishes between surface adherence and ingestion
- 1 November 1979
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 12 (4) , 459-467
- https://doi.org/10.1099/00222615-12-4-459
Abstract
Polymorphonuclear leukocytes [rabbit] selectively inhibited the incorporation of 14C-uridine by intracellular staphylococci. Within specified limits, the amount of radiolabel incorporated by extracellular staphylococci was related to bacterial concentration. Incorporation of labeled uridine can be exploited to assay the extent to which association between staphylococci and polymorphonuclear leukocytes reflects surface adherence as opposed to ingestion. A comparison of the new method with a conventional viable-count determination of leukocyte-associated bacteria shows it is comparable in efficiency when non-immune serum is used as opsonin and superior when specific opsonin is used.This publication has 4 references indexed in Scilit:
- Quantitative measurement of phagocytosis of Neisseria gonorrhoeae by mouse peritoneal macrophagesInfection and Immunity, 1978
- NEW SEMI-QUANTITATIVE RADIOMETRIC OPSONIN ASSAY - SELECTIVE MEASUREMENT OF OPSONIZING CAPACITY OF ALTERNATIVE PATHWAY1978
- Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: A quantitative assay using [3H] thymidine labeled bacteriaJournal of Immunological Methods, 1977
- Penetration of Penicillin into Human Phagocytes Containing Neisseria gonorrhoeae: Intracellular Survival and Growth at Optimum Concentrations of AntibioticJournal of General Microbiology, 1976