STUDIES ON CARBOHYDRATE-METABOLIZING ENZYMES Part XXV. THE DEBRANCHING ENZYME SYSTEM IN GERMINATED BARLEY

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Abstract
Extracts of germinated barley contain two debranching enzymes which have been separated from each other, and from α-amylase, by continuous electrophoresis followed by gel filtration. Amylopectin 6-glucanohydrolase hydrolyses the outer-most inter-chain linkages in amylopectin but has no action on glycogen or on α-limit dextrins. Limit dextrinase has no action on amylopectin, but readily hydrolyses α-limit dextrins; it differs fron amylopectin 6-glucanohydrolase in having a relatively lower activity at pH 4, pH 7 or 60° C, and is selectively activated by chloride ions.