Structure of a Sugar Chain of a Protease Inhibitor Isolated from Barbados Pride (Caesalpinia pulcherrima Sw.) Seeds
- 1 July 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 100 (1) , 1-10
- https://doi.org/10.1093/oxfordjournals.jbchem.a121681
Abstract
An asparagine-linked sugar chain of a protease inhibitor from barbados pride (Caesalpinia pulcherrima Sw.) was liberated by hydrazinolysis. After iV-acetylation, the reducing end residue of this carbohydrate unit was coupled with 2-aminopyridine and the pyridylamino (PA-) derivative was purified by gel-filtration and reversed-phase HPLC. The structure of the resulting PA-sugar chain was determined mainly by stepwise exoglycosidase digestions and 500 MHz 1H-NMR spectroscopy and proved to be as follows:This publication has 2 references indexed in Scilit:
- Structure of a complex-type sugar chain of human glycophorin ABiochemistry, 1981
- The structure of the glycan moiety of tora-bean (phaseolus vulgaris)lectinCarbohydrate Research, 1980