MECHANISM OF ACTION OF THE LACTIC DEHYDROGENASE OF THE MAMMALIAN ERYTHROCYTE: I. INFLUENCE OF INHIBITORS

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Abstract
This paper pertains to the inhibition of lactic dehydrogenase by substances structurally related to its substrates, pyruvate and lactate. Oxalate, tartronate, and malonate inhibit the enxyme in a competitive manner towards lactate, and in a noncompeti-tive manner towards pyruvate. Phenoxyactate causes simultaneous competitive and noncompetitive inhibition towards both lactate and pyruvate. The results obtained lead to the conclusion that, for their activation, pyruvate and lactate react with different sites on the enzyme surface.