Centromere: Absence of DNA Replication during Chromatid Separation in Human Fibroblasts
- 16 December 1966
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 154 (3755) , 1463-1464
- https://doi.org/10.1126/science.154.3755.1463
Abstract
Cultures of human fibroblasts were labeled briefly with tritiated thymidine and fixed; autoradiographs were made and exposed for 3½ months. No labeling was noted over the centromere of metaphase or anaphase chromosomes. The technique was sensitive to replication at the centromere of a DNA helix only 2.5 microns long, considerably shorter than the estimated length of a replicon in humans. This suggests that chromatid separation during mitosis is not associated with delayed replication of a short segment of chromosomal DNA.This publication has 7 references indexed in Scilit:
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