MICELLES OF CEREBROSIDE SULPHATE

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Abstract
The critical micelle concentration of cerebroside sulphate in water is 001 mM: it increases with increasing concentrations of buffer to 007 mM in 01 M sodium acetate and formate buffers. pH 56 and 45 respectively. The partial specific volume of the micelles is about 094. The behaviour of the micelles in the ultracentrifuge and on Sephadex G-200 shows them to he grossly heterogeneous with respect to size. In 01 M buffer s 20, w is about 26 S; in water or 001 M buffer smaller micelles with an s20, w. of about 6 S are also present. In 001 M formate, pH 45, the smallest species detectable by equilibrium ultracentrifugation had a micellar weight of about 180,000 corresponding to an aggregation number of about 180. Much larger aggregates were also present. It is suggested that the smallest micelles are the substrate for sulphatase A when this is acting as a cerebroside sulphatase in buffers of low ionic strength.

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