MTS Interferon Assay: A Simplified Cellular Dehydrogenase Assay for Interferon Activity Using a Water-Soluble Tetrazolium Salt
- 1 January 1996
- journal article
- Published by Mary Ann Liebert Inc in Journal of Interferon & Cytokine Research
- Vol. 16 (1) , 31-33
- https://doi.org/10.1089/jir.1996.16.31
Abstract
MTS, a tetrazolium dye, is reduced by hydrogenases in living cells to a water-soluble formazan. When it is added to the medium at the end of a cytopathic effects (CPE) inhibition interferon assay, the formazan formed diffuses into the medium; the resultant optical density directly and quantitatively measures how much cellular damage has been produced by the challenge virus in the presence of different amounts of interferon. The use of MTS has considerable advantages in that after it is added, no further steps, such as washing of the cells, extraction of dye, or other manipulations, are needed.Keywords
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