COOPERATIVITY PATTERN IN THE INTERACTION OF THE ANTI-ESTROGEN DRUG CLOMIPHENE WITH THE MUSCARINIC RECEPTORS
- 1 January 1982
- journal article
- research article
- Vol. 21 (2) , 287-293
Abstract
The possible interaction between the muscarinic receptors and the antiestrogenic drug clomiphene citrate was investigated in competition experiments using the highly specific antagonist 3H-N-methyl-4-piperidyl benzilate (4-NMPB) in various regions of rat brain. Clomiphene can displace muscarinic antagonists from their receptor-ligand complexes. Binding analyses and determinations of the kinetics of dissociation of [3H]4-NMPB-receptor complexes indicate the binding of more than 1 molecule of clomiphene in a positively cooperative pattern. The nonsteroidal antiestrogenic drugs, e.g., clomiphene, may exert their effects not only through the specific estrogen receptor but also in conjunction with muscarinic system.This publication has 6 references indexed in Scilit:
- Molecular regulation of receptors: interaction of beta-estradiol and progesterone with the muscarinic system.Proceedings of the National Academy of Sciences, 1981
- CHARACTERIZATION OF MUSCARINIC ACETYLCHOLINE RECEPTORS FROM MOUSE-BRAIN - EVIDENCE FOR REGIONAL HETEROGENEITY AND ISOMERIZATION1979
- Induction of Ovulation with Clomiphene CitrateFertility and Sterility, 1979
- Studies on muscarinic acetylcholine receptors from mouse brain: Characterization of the interaction with antagonistsBrain Research, 1978
- BINDING OF ANTAGONISTS TO BRAIN MUSCARINIC RECEPTORS1978
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951