The Improvement of Spinach Growth by Nano-anatase TiO2 Treatment Is Related to Nitrogen Photoreduction

The improvement of spinach growth is proved to relate to N₂ fixation by nano-anatase TiO₂ in this study. The results show that all spinach leaves kept green by nano-anatase TiO₂ treatment and all old leaves of control turned yellow white under culture with N-deficient solution. And the fresh weight, dry weight, and contents of total nitrogen, \( {\text{NH}}^{ + }_{4} \), chlorophyll, and protein of spinach by nano-anatase TiO₂ treatment presented obvious enhancement compared with control. Whereas the improvements of yield of spinach were not as good as nano-anatase TiO₂ treatment under N-deficient condition, confirming that nano-anatase TiO₂ on exposure to sunlight could chemisorb N₂ directly or reduce N₂ to NH₃ in the spinach leaves, transforming into organic nitrogen and improving the growth of spinach. Bulk TiO₂ effect, however, was not as significant as nano-anatase TiO₂. A possible metabolism of the function of nano-anatase TiO₂ reducing N₂ to NH₃ was discussed.